Expression of the Thermobifida fusca xylanase Xyn11A in Pichia pastoris and its characterization.

ABSTRACT:

Background

Xylan is a major component of plant cells and the most abundant hemicellulose. Xylanases degrade xylan into monomers by randomly cleaving β-1,4-glycosidic bonds in the xylan backbone, and have widespread potential applications in various industries. The purpose of our study was to clone and express the endoxylanase gene xynA of Thermobifida fusca YX in its native form and with a C-terminal histidine (His) tag in Pichia pastoris X-33. We analyzed and compared these two forms of the protein and examined their potential applications in various industries.

Results

The xynA gene from T. fusca YX was successfully cloned and expressed using P. pastoris X-33. We produced a recombinant native form of the protein (rXyn11A) and a C-terminal His-tagged form of the desired pro

SUBMITTER: Zhao L 

PROVIDER: S-EPMC4369062 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature