Crucial role of androgen receptor in vascular H2S biosynthesis induced by testosterone.

ABSTRACT:

Background and purpose

Hydrogen sulphide (H2S) is a gaseous mediator strongly involved in cardiovascular homeostasis, where it provokes vasodilatation. Having previously shown that H2 S contributes to testosterone-induced vasorelaxation, here we aim to uncover the mechanisms underlying this effect.

Experimental approach

H2 S biosynthesis was evaluated in rat isolated aortic rings following androgen receptor (NR3C4) stimulation. Co-immunoprecipitation and surface plasmon resonance analysis were performed to investigate mechanisms involved in NR3C4 activation.

Key results

Pretreatment with NR3C4 antagonist nilutamide prevented testosterone-induced increase in H2S and reduced its vasodilator effect. Androgen agonist mesterolone also increased H2S and induced vasodilatati

SUBMITTER: Brancaleone V 

PROVIDER: S-EPMC4369260 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature