Interactions of hydrogen sulfide with myeloperoxidase.

ABSTRACT:

Background and purpose

The actions of hydrogen sulfide in human physiology have been extensively studied and, although it is an essential mediator of many biological functions, the underlying molecular mechanisms of its actions are ill-defined. To elucidate the roles of sulfide in inflammation, we have investigated its interactions with human myeloperoxidase (MPO), a major contributor to inflammatory oxidative stress.

Experimental approach

The interactions of sulfide and MPO were investigated using electron paramagnetic resonance, electronic circular dichroism, UV-vis and stopped-flow spectroscopies.

Key results

We found favourable reactions between sulfide and the native-ferric enzyme as well as the MPO redox intermediates, ferrous MPO, compound I and compound II. Su

SUBMITTER: Palinkas Z 

PROVIDER: S-EPMC4369261 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature