Plasmodium knowlesi gene expression differs in ex vivo compared to in vitro blood-stage cultures.

ABSTRACT:

Background

Plasmodium knowlesi is one of five Plasmodium species known to cause malaria in humans and can result in severe illness and death. While a zoonosis in humans, this simian malaria parasite species infects macaque monkeys and serves as an experimental model for in vivo, ex vivo and in vitro studies. It has underpinned malaria discoveries relating to host-pathogen interactions, the immune response and immune evasion strategies. This study investigated differences in P. knowlesi gene expression in samples from ex vivo and in vitro cultures.

Methods

Gene expression profiles were generated using microarrays to compare the stage-specific transcripts detected for a clone of P. knowlesi propagated in the blood of a rhesus macaque host and then grown in an ex-vivo culture,

SUBMITTER: Lapp SA 

PROVIDER: S-EPMC4369371 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature