Establishment of a proteome profile and identification of molecular markers for mouse spermatogonial stem cells.

ABSTRACT: Spermatogonial stem cells (SSCs) are undifferentiated cells that are required to maintain spermatogenesis throughout the reproductive life of mammals. Although SSC transplantation and culture provide a powerful tool to identify the mechanisms regulating SSC function, the precise signalling mechanisms governing SSC self-renewal and specific surface markers for purifying SSCs remain to be clearly determined. In the present study, we established a steady SSC culture according to the method described by Shinohara's lab. Fertile progeny was produced after transplantation of cultured SSCs into infertile mouse testis, and the red fluorescence exhibited by the culture cell membranes was stably and continuously transmitted to the offspring. Next, via advanced mass spectrometry and an optimized prot

SUBMITTER: Zhou Q 

PROVIDER: S-EPMC4369810 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature