P2Y12 expression and function in alternatively activated human microglia.

ABSTRACT:

Objective

To investigate and measure the functional significance of altered P2Y12 expression in the context of human microglia activation.

Methods

We performed in vitro and in situ experiments to measure how P2Y12 expression can influence disease-relevant functional properties of classically activated (M1) and alternatively activated (M2) human microglia in the inflamed brain.

Results

We demonstrated that compared to resting and classically activated (M1) human microglia, P2Y12 expression is increased under alternatively activated (M2) conditions. In response to ADP, the endogenous ligand of P2Y12, M2 microglia have increased ligand-mediated calcium responses, which are blocked by selective P2Y12 antagonism. P2Y12 antagonism was also shown to decrease migratory and in

SUBMITTER: Moore CS 

PROVIDER: S-EPMC4370387 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature