Identification of reference genes for real-time quantitative PCR experiments in the liverwort Marchantia polymorpha.

ABSTRACT: Real-time quantitative polymerase chain reaction (qPCR) has become widely used as a method to compare gene transcript levels across different conditions. However, selection of suitable reference genes to normalize qPCR data is required for accurate transcript level analysis. Recently, Marchantia polymorpha has been adopted as a model for the study of liverwort development and land plant evolution. Identification of appropriate reference genes has therefore become a necessity for gene expression studies. In this study, transcript levels of eleven candidate reference genes have been analyzed across a range of biological contexts that encompass abiotic stress, hormone treatment and different developmental stages. The consistency of transcript levels was assessed using both geNorm and NormFind

SUBMITTER: Saint-Marcoux D 

PROVIDER: S-EPMC4370483 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature