Conjoined use of EM and NMR in RNA structure refinement.

ABSTRACT: More than 40% of the RNA structures have been determined using nuclear magnetic resonance (NMR) technique. NMR mainly provides local structural information of protons and works most effectively on relatively small biomacromolecules. Hence structural characterization of large RNAs can be difficult for NMR alone. Electron microscopy (EM) provides global shape information of macromolecules at nanometer resolution, which should be complementary to NMR for RNA structure determination. Here we developed a new energy term in Xplor-NIH against the density map obtained by EM. We conjointly used NMR and map restraints for the structure refinement of three RNA systems—U2/U6 small-nuclear RNA, genome-packing motif (Ψ(CD))2 from Moloney murine leukemia virus, and ribosome-binding element from turnip cr

SUBMITTER: Gong Z 

PROVIDER: S-EPMC4370883 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature