Multifactorial interaction of growth factors on Penaeus monodon lymphoid cells and the impact of IGFs in DNA synthesis and metabolic activity in vitro.

ABSTRACT: Development of continuous cell lines from shrimp is essential to investigate viral pathogens. Unfortunately, there is no valid cell line developed from crustaceans in general and shrimps in particular to address this issue. Lack of information on the requirements of cells in vitro limits the success of developing a cell line, where the microenvironment of a cell culture, provided by the growth medium, is of prime importance. Screening and optimization of growth medium components based on statistical experimental designs have been widely used for improving the efficacy of cell culture media. Accordingly, we applied Plackett-Burman design and response surface methodology to study multifactorial interactions between the growth factors in shrimp cell culture medium and to identify the most imp

SUBMITTER: Jayesh P 

PROVIDER: S-EPMC4371564 | biostudies-literature | 2015 May

REPOSITORIES: biostudies-literature