Quantitative imaging of glutathione in live cells using a reversible reaction-based ratiometric fluorescent probe.

ABSTRACT: Glutathione (GSH) plays an important role in maintaining redox homeostasis inside cells. Currently, there are no methods available to quantitatively assess the GSH concentration in live cells. Live cell fluorescence imaging revolutionized the field of cell biology and has become an indispensable tool in current biological studies. In order to minimize the disturbance to the biological system in live cell imaging, the probe concentration needs to be significantly lower than the analyte concentration. Because of this, any irreversible reaction-based GSH probe can only provide qualitative results within a short reaction time and will exhibit maximum response regardless of the GSH concentration if the reaction is completed. A reversible reaction-based probe with an appropriate equilibrium cons

SUBMITTER: Jiang X 

PROVIDER: S-EPMC4371605 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature