TGFβ and Wnt in cardiac outflow tract defects in offspring of diabetic pregnancies.

ABSTRACT:

Background

Diabetes mellitus in pregnancy causes defects in infant heart, including the outflow tracts (OFTs). Development of the aorta and pulmonary artery, which are derived from the common OFT in the embryo, is regulated by the transforming growth factor β (TGFβ) and Wnt families, and can be perturbed by hyperglycemia-generated intracellular stress conditions. However, the underlying cellular and molecular mechanisms remain to be delineated.

Methods

Female mice were induced diabetic with streptozotocin. Embryonic and fetal OFTs were examined morphologically and histologically. Cell proliferation was assessed using 5'-bromo-2'-deoxyuridine incorporation assay. Oxidative and endoplasmic reticulum (ER) stress markers and TGFβ factors were detected using immunohistochemistry.

SUBMITTER: Zhao Z 

PROVIDER: S-EPMC4372991 | biostudies-literature | 2014 Oct

REPOSITORIES: biostudies-literature