Identification and quantitative mRNA analysis of a novel splice variant of GPIHBP1 in dairy cattle.

ABSTRACT:

Background

Identification of functional genes affecting milk production traits is very crucial for improving breeding efficiency in dairy cattle. Many potential candidate genes have been identified through our previous genome wide association study (GWAS). Of these, GPIHBP1 is an important novel candidate gene for milk production traits. However, the mRNA structure of the bovine GPIHBP1 gene is not fully determined up to now.

Results

In this study, we identified a novel alternatively splice transcript variant (X5) which leads to a 31 bp insertion in exon 3 and also confirmed the other four existed transcripts (X1, X2, X3 and X4) of the bovine GPIHBP1 gene. We showed that transcript X5 with a 31 bp insertion and transcript X1 with an 8 bp deletion might have tremendous effect

SUBMITTER: Yang J 

PROVIDER: S-EPMC4373091 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature