Transcriptional analysis of micro-dissected articular cartilage in post-traumatic murine osteoarthritis.

ABSTRACT:

Objective

Identify gene changes in articular cartilage of the medial tibial plateau (MTP) at 2, 4 and 8 weeks after destabilisation of the medial meniscus (DMM) in mice. Compare our data with previously published datasets to ascertain dysregulated pathways and genes in osteoarthritis (OA).

Design

RNA was extracted from the ipsilateral and contralateral MTP cartilage, amplified, labelled and hybridized on Illumina WGv2 microarrays. Results were confirmed by real-time polymerase chain reaction (PCR) for selected genes.

Results

Transcriptional analysis and network reconstruction revealed changes in extracellular matrix and cytoskeletal genes induced by DMM. TGFβ signalling pathway and complement and coagulation cascade genes were regulated at 2 weeks. Fibronectin (Fn1) i

SUBMITTER: Gardiner MD 

PROVIDER: S-EPMC4373757 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature