Multiplicity of steady states in glycolysis and shift of metabolic state in cultured mammalian cells.

ABSTRACT: Cultured mammalian cells exhibit elevated glycolysis flux and high lactate production. In the industrial bioprocesses for biotherapeutic protein production, glucose is supplemented to the culture medium to sustain continued cell growth resulting in the accumulation of lactate to high levels. In such fed-batch cultures, sometimes a metabolic shift from a state of high glycolysis flux and high lactate production to a state of low glycolysis flux and low lactate production or even lactate consumption is observed. While in other cases with very similar culture conditions, the same cell line and medium, cells continue to produce lactate. A metabolic shift to lactate consumption has been correlated to the productivity of the process. Cultures that exhibited the metabolic shift to lactate consump

SUBMITTER: Mulukutla BC 

PROVIDER: S-EPMC4373774 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature