P38 MAP Kinase Inhibition Reduces Propionibacterium acnes-Induced Inflammation in Vitro.

ABSTRACT:

Introduction

Propionibacterium acnes, a ubiquitous skin bacterium, stimulates keratinocytes to produce a number of proinflammatory cytokines and may contribute to inflammatory acne. The aim of the study was to investigate whether P. acnes-induced proinflammatory cytokine release is mediated by P. acnes-induced activation of p38 mitogen-activated protein kinase (p38 MAPK or p38) in human keratinocytes.

Methods

Immunohistochemistry was used to evaluate p38 phosphorylation in human skin samples with or without acne. Primary human keratinocytes and epidermal skin equivalents were exposed to viable P. acnes. Phosphorylation of MAPKs without or with p38 inhibitors was examined by Western blot and cytokine secretion was detected by Enzyme-Linked Immunosorbent Assay (ELISA).

Resu

SUBMITTER: Li WH 

PROVIDER: S-EPMC4374066 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature