Bacterial and viral identification and differentiation by amplicon sequencing on the MinION nanopore sequencer.

ABSTRACT:

Background

The MinION™ nanopore sequencer was recently released to a community of alpha-testers for evaluation using a variety of sequencing applications. Recent reports have tested the ability of the MinION™ to act as a whole genome sequencer and have demonstrated that nanopore sequencing has tremendous potential utility. However, the current nanopore technology still has limitations with respect to error-rate, and this is problematic when attempting to assemble whole genomes without secondary rounds of sequencing to correct errors. In this study, we tested the ability of the MinION™ nanopore sequencer to accurately identify and differentiate bacterial and viral samples via directed sequencing of characteristic genes shared broadly across a target clade.

Results

Using a 6 h

SUBMITTER: Kilianski A 

PROVIDER: S-EPMC4374364 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature