Genome-wide identification of evolutionarily conserved alternative splicing events in flowering plants.

ABSTRACT: Alternative splicing (AS) plays important roles in many plant functions, but its conservation across the plant kingdom is not known. We describe a methodology to identify AS events and identify conserved AS events across large phylogenetic distances using RNA-Seq datasets. We applied this methodology to transcriptome data from nine angiosperms including Amborella, the single sister species to all other extant flowering plants. AS events within 40-70% of the expressed multi-exonic genes per species were found, 27,120 of which are conserved among two or more of the taxa studied. While many events are species specific, many others are shared across long evolutionary distances suggesting they have functional significance. Conservation of AS event data provides an estimate of the number of ance

SUBMITTER: Chamala S 

PROVIDER: S-EPMC4374538 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature