Markedly increased IP-10 production by blood-brain barrier in neuromyelitis optica.

ABSTRACT:

Objective

Severe damage to the blood-brain barrier (BBB) allows anti-aquaporin 4 (AQP4) antibodies to access the astrocytic endfeet in neuromyelitis optica (NMO). In the current study, we identified the pathogenic cytokines/chemokines that are responsible for the BBB malfunction induced by NMO sera.

Methods

We measured the levels of 27 cytokines/chemokines in human brain microvascular endothelial cells (BMECs) after exposure to sera obtained from patients with the acute and stable phases of anti-AQP4 antibody-positive NMO spectrum disorder (NMOSD), multiple sclerosis (MS) patients and healthy controls (HC) using a multiplexed fluorescent bead-based immunoassay system.

Results

The induced protein (IP)-10 level in the cells was markedly increased following exposure to a

SUBMITTER: Shimizu F 

PROVIDER: S-EPMC4374747 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature