Genome-based identification of active prophage regions by next generation sequencing in Bacillus licheniformis DSM13.

ABSTRACT: Prophages are viruses, which have integrated their genomes into the genome of a bacterial host. The status of the prophage genome can vary from fully intact with the potential to form infective particles to a remnant state where only a few phage genes persist. Prophages have impact on the properties of their host and are therefore of great interest for genomic research and strain design. Here we present a genome- and next generation sequencing (NGS)-based approach for identification and activity evaluation of prophage regions. Seven prophage or prophage-like regions were identified in the genome of Bacillus licheniformis DSM13. Six of these regions show similarity to members of the Siphoviridae phage family. The remaining region encodes the B. licheniformis orthologue of the PBSX prophage

SUBMITTER: Hertel R 

PROVIDER: S-EPMC4374763 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature