Proteomic profile of an acute partial bladder outlet obstruction.

ABSTRACT:

Introduction

Partial bladder outlet obstruction (pBOO) is a ubiquitous problem in urology. From posterior urethral valves to prostatic hypertrophy, pBOO results in significant morbidity and mortality. However, the pathophysiology is not completely understood. Proteomics uses mass spectrometry to accurately quantify change in tissue protein concentration. Therefore, we have applied proteomic analysis to a rodent model to assess for protein changes after a surgically induced pBOO. We hypothesize that proteomic analysis after an acute obstruction will determine the most prevalent initial protein response and, potentially, novel molecular pathways.

Methods

Sprague Dawley rats underwent a surgically induced pBOO (n = 3 per group) for 3, 7, or 14 days. Bladders were assessed for w

SUBMITTER: Alsaikhan B 

PROVIDER: S-EPMC4374996 | biostudies-literature | 2015 Mar-Apr

REPOSITORIES: biostudies-literature