SAMP1/YitFc mice develop ileitis via loss of CCL21 and defects in dendritic cell migration.

ABSTRACT:

Background & aims

The lymphatic chemokine CCL21 is required for dendritic cell (DC) migration from tissues to lymph nodes, which helps establish tolerance to foreign yet harmless antigens. We demonstrate that CCL21 is almost completely absent from SAMP1/YitFc (SAMP) mice, which spontaneously develop chronic ileitis that resembles Crohn's disease, and that DC migration is severely impaired in these mice compared with AKR mice (controls). Toll-like receptor agonists like the Toll-like receptor 7 agonist R848 induce DC maturation and mobilization.

Methods

We collected intestinal and other tissues and mesenteric lymph nodes (MLN) from SAMP mice. Expression of CCL21 was measured by quantitative reverse transcription polymerase chain reaction and immunofluorescence analyses; spont

SUBMITTER: Mikulski Z 

PROVIDER: S-EPMC4375031 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature