Culture-independent evaluation of nonenveloped-virus infectivity reduced by free-chlorine disinfection.

ABSTRACT: The inability of molecular detection methods to distinguish disinfected virions from infectious ones has hampered the assessment of infectivity for enteric viruses caused by disinfection practices. In the present study, the reduction of infectivity of murine norovirus S7-PP3 and mengovirus vMC0, surrogates of human noroviruses and enteroviruses, respectively, caused by free-chlorine treatment was characterized culture independently by detecting carbonyl groups on viral capsid protein. The amount of carbonyls on viral capsid protein was evaluated by the proportion of biotinylated virions trapped by avidin-immobilized gel (percent adsorbed). This culture-independent approach demonstrated that the percent adsorbed was significantly correlated with the logarithm of the infectious titer of test

SUBMITTER: Sano D 

PROVIDER: S-EPMC4375320 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature