High-throughput microfluidic method to study biofilm formation and host-pathogen interactions in pathogenic Escherichia coli.

ABSTRACT: Biofilm formation and host-pathogen interactions are frequently studied using multiwell plates; however, these closed systems lack shear force, which is present at several sites in the host, such as the intestinal and urinary tracts. Recently, microfluidic systems that incorporate shear force and very small volumes have been developed to provide cell biology models that resemble in vivo conditions. Therefore, the objective of this study was to determine if the BioFlux 200 microfluidic system could be used to study host-pathogen interactions and biofilm formation by pathogenic Escherichia coli. Strains of various pathotypes were selected to establish the growth conditions for the formation of biofilms in the BioFlux 200 system on abiotic (glass) or biotic (eukaryotic-cell) surfaces. Biofilm

SUBMITTER: Tremblay YD 

PROVIDER: S-EPMC4375333 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature