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A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging.

ABSTRACT: We describe a microfluidic device designed specifically for the reversible immobilisation of Schizosaccharomyces pombe (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. Cells are held over several hours and can continue to grow and divide within the device during fluorescence imaging.

SUBMITTER: Bell L

PROVIDER: S-EPMC4375559 | biostudies-literature | 2014 Mar

REPOSITORIES: biostudies-literature

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A microfluidic device for the hydrodynamic immobilisation of living fission yeast cells for super-resolution imaging.

Bell Laurence L Seshia Ashwin A Lando David D Laue Ernest E Palayret Matthieu M Lee Steven F SF Klenerman David D

Sensors and actuators. B, Chemical 20140301

We describe a microfluidic device designed specifically for the reversible immobilisation of <i>Schizosaccharomyces pombe</i> (Fission Yeast) cells to facilitate live cell super-resolution microscopy. Photo-Activation Localisation Microscopy (PALM) is used to create detailed super-resolution images within living cells with a modal accuracy of >25 nm in the lateral dimensions. The novel flow design captures and holds cells in a well-defined array with minimal effect on the normal growth kinetics. ...[more]

PMID: 25844024

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