Sequence of gating charge movement and pore gating in HERG activation and deactivation pathways.

ABSTRACT: KV11.1 voltage-gated K(+) channels are noted for unusually slow activation, fast inactivation, and slow deactivation kinetics, which tune channel activity to provide vital repolarizing current during later stages of the cardiac action potential. The bulk of charge movement in human ether-a-go-go-related gene (hERG) is slow, as is return of charge upon repolarization, suggesting that the rates of hERG channel opening and, critically, that of deactivation might be determined by slow voltage sensor movement, and also by a mode-shift after activation. To test these ideas, we compared the kinetics and voltage dependence of ionic activation and deactivation with gating charge movement. At 0 mV, gating charge moved ∼threefold faster than ionic current, which suggests the presence of additional sl

SUBMITTER: Goodchild SJ 

PROVIDER: S-EPMC4375626 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature