Modes of diffusion of cholera toxin bound to GM1 on live cell membrane by image mean square displacement analysis.

ABSTRACT: The image-mean square displacement technique applies the calculation of the mean square displacement commonly used in single-molecule tracking to images without resolving single particles. The image-mean square displacement plot obtained is similar to the mean square displacement plot obtained using the single-particle tracking technique. This plot is then used to reconstruct the protein diffusion law and to identify whether the labeled molecules are undergoing pure isotropic, restricted, corralled, transiently confined, or directed diffusion. In our study total internal reflection fluorescence microscopy images were taken of Cholera toxin subunit B (CtxB) membrane-labeled NIH 3T3 mouse fibroblasts and MDA 231 MB cells. We found a population of CTxB undergoing purely isotropic diffusion an

SUBMITTER: Moens PDJ 

PROVIDER: S-EPMC4375681 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature