A transposon-mediated system for flexible control of transgene expression in stem and progenitor-derived lineages.

ABSTRACT: Precise methods for transgene regulation are important to study signaling pathways and cell lineages in biological systems where gene function is often recycled within and across lineages. We engineered a genetic toolset for flexible transgene regulation in these diverse cellular contexts. Specifically, we created an optimized piggyBac transposon-based system, allowing for the facile generation of stably transduced cell lineages in vivo and in vitro. The system, termed pB-Tet-GOI (piggyBac-transposable tetracycline transactivator-mediated flexible expression of a genetic element of interest), incorporates the latest generation of tetracycline (Tet) transactivator and reverse Tet transactivator variants--along with engineered mutants--in order to provide regulated transgene expression upon

SUBMITTER: Akhtar AA 

PROVIDER: S-EPMC4375828 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature