Cyclooxygenase 1 mRNA expression is undetectable in Madin Darby Canine Kidney cells.

ABSTRACT:

Background

Madin Darby Canine Kidney (MDCK) cells form polarized epithelium in vitro and are routinely used in research fields ranging from protein trafficking to influenza. However, the canine origin of these cells also means that compared to man or mouse, genomic resources are more limited and performance of commercially available antibodies often untested. The synthesis of pro-inflammatory prostaglandins in the kidney is mediated by the constitutively expressed cyclooxygenase 1 and the inducible cyclooxygenase 2 (COX-1 and COX-2, respectively). There are conflicting reports on the expression of COX-1 and COX-2 in MDCK cells and this lingering uncertainty about such important pharmacological targets may affect the interpretation of results obtained from this cell line.

Results

SUBMITTER: Pelletier G 

PROVIDER: S-EPMC4375849 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature