Identification of oxidized phospholipids in bronchoalveolar lavage exposed to low ozone levels using multivariate analysis.

ABSTRACT: Chemical reactions with unsaturated phospholipids in the respiratory tract lining fluid have been identified as one of the first important steps in the mechanisms mediating environmental ozone toxicity. As a consequence of these reactions, complex mixtures of oxidized lipids are generated in the presence of mixtures of non-oxidized naturally occurring phospholipid molecular species, which challenge methods of analysis. Untargeted mass spectrometry and statistical methods were employed to approach these complex spectra. Human bronchoalveolar lavage (BAL) was exposed to low levels of ozone, and samples with and without derivatization of aldehydes were analyzed by liquid chromatography electrospray ionization tandem mass spectrometry. Data processing was carried out using principal component

SUBMITTER: Almstrand AC 

PROVIDER: S-EPMC4375957 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature