Measurement of bisphenol A, bisphenol A ß-D-glucuronide, genistein, and genistein 4'-ß-D-glucuronide via SPE and HPLC-MS/MS.

ABSTRACT: Bisphenol A (BPA) is a synthetic industrial reactant used in the production of polycarbonate plastics, and genistein is a natural phytoestrogen abundant in the soybean. Current studies investigating the endocrine-disrupting effects of concomitant exposures to BPA and genistein have warranted the development of an analytical method for the simultaneous measurement of BPA and genistein, as well as their primary metabolites, bisphenol A ß-D-glucuronide (BPA gluc) and genistein 4'-ß-D-glucuronide (genistein gluc), respectively. All four analytes were extracted from rat plasma via solid phase extraction (SPE). Three SPE cartridges and four elution schemes were tested. Plasma extraction using Bond Elut Plexa cartridges with sequential addition of ethyl acetate, methanol, and acetonitrile yielded

SUBMITTER: Coughlin JL 

PROVIDER: S-EPMC4376255 | biostudies-literature | 2011 Aug

REPOSITORIES: biostudies-literature