Reproducibility and reliability assays of the gene expression-measurements.

ABSTRACT:

Background

Reliability and reproducibility are key metrics for gene expression assays. This report assesses the utility of the correlation coefficient in the analysis of reproducibility and reliability of gene expression data.

Results

The correlation coefficient alone is not sufficient to assess equality among sample replicates but when coupled with slope and scatter plots expression data equality can be better assessed. Narrow-intervals of scatter plots should be shown as a tool to inspect the actual level of noise within the data. Here we propose a method to examine expression data reproducibility, which is based on the ratios of both the means and the standard deviations for the inter-treatment expression ratios of genes. In addition, we introduce a fold-change threshold

SUBMITTER: Darbani B 

PROVIDER: S-EPMC4376515 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature