Extracellular vesicle-depleted fetal bovine and human sera have reduced capacity to support cell growth.

ABSTRACT:

Background

Fetal bovine serum (FBS) is the most widely used serum supplement for mammalian cell culture. It supports cell growth by providing nutrients, growth signals, and protection from stress. Attempts to develop serum-free media that support cell expansion to the same extent as serum-supplemented media have not yet succeeded, suggesting that FBS contains one or more as-yet-undefined growth factors. One potential vehicle for the delivery of growth factors from serum to cultured cells is extracellular vesicles (EVs).

Methods

EV-depleted FBS and human serum were generated by 120,000g centrifugation, and its cell growth-supporting activity was measured. Isolated EVs from FBS were quantified and characterized by nanoparticle tracking analysis, electron microscopy, and protei

SUBMITTER: Eitan E 

PROVIDER: S-EPMC4376846 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature