Identification of tapetum-specific genes by comparing global gene expression of four different male sterile lines in Brassica oleracea.

ABSTRACT: The tapetum plays an important role in anther development by providing necessary enzymes and nutrients for pollen development. However, it is difficult to identify tapetum-specific genes on a large-scale because of the difficulty of separating tapetum cells from other anther tissues. Here, we reported the identification of tapetum-specific genes by comparing the gene expression patterns of four male sterile (MS) lines of Brassica oleracea. The abortive phenotypes of the four MS lines revealed different defects in tapetum and pollen development but normal anther wall development when observed by transmission electron microscopy. These tapetum displayed continuous defective characteristics throughout the anther developmental stages. The transcriptome from flower buds, covering all anther dev

SUBMITTER: Ma Y 

PROVIDER: S-EPMC4377141 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature