HT-29 and Caco-2 reporter cell lines for functional studies of nuclear factor kappa B activation.

ABSTRACT: The NF-κB is a transcription factor which plays a key role in regulating biological processes. In response to signals, NF-κB activation occurs via phosphorylation of its inhibitor, which dissociates from the NF-κB dimer allowing the translocation to the nucleus, inducing gene expression. NF-κB activation has direct screening applications for drug discovery for several therapeutic indications. Thus, pathway-specific reporter cell systems appear as useful tools to screen and unravel the mode of action of probiotics and natural and synthetic compounds. Here, we describe the generation, characterization, and validation of human epithelial reporter cell lines for functional studies of NF-κB activation by different pro- and anti-inflammatory agents. Caco-2 and HT-29 cells were transfected with a

SUBMITTER: Mastropietro G 

PROVIDER: S-EPMC4377483 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature