Opposite rheological properties of neuronal microcompartments predict axonal vulnerability in brain injury.

ABSTRACT: Although pathological changes in axonal morphology have emerged as important features of traumatic brain injury (TBI), the mechanical vulnerability of the axonal microcompartment relative to the cell body is not well understood. We hypothesized that soma and neurite microcompartments exhibit distinct mechanical behaviors, rendering axons more sensitive to a mechanical injury. In order to test this assumption, we combined protein micropatterns with magnetic tweezer rheology to probe the viscoelastic properties of neuronal microcompartments. Creep experiments revealed two opposite rheological behaviors within cortical neurons: the cell body was soft and characterized by a solid-like response, whereas the neurite compartment was stiffer and viscous-like. By using pharmacological agents, we de

SUBMITTER: Grevesse T 

PROVIDER: S-EPMC4377573 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature