An improved genome of the model marine alga Ostreococcus tauri unfolds by assessing Illumina de novo assemblies.

ABSTRACT:

Background

Cost effective next generation sequencing technologies now enable the production of genomic datasets for many novel planktonic eukaryotes, representing an understudied reservoir of genetic diversity. O. tauri is the smallest free-living photosynthetic eukaryote known to date, a coccoid green alga that was first isolated in 1995 in a lagoon by the Mediterranean sea. Its simple features, ease of culture and the sequencing of its 13 Mb haploid nuclear genome have promoted this microalga as a new model organism for cell biology. Here, we investigated the quality of genome assemblies of Illumina GAIIx 75 bp paired-end reads from Ostreococcus tauri, thereby also improving the existing assembly and showing the genome to be stably maintained in culture.

Results

The 3 asse

SUBMITTER: Blanc-Mathieu R 

PROVIDER: S-EPMC4378021 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature