Unbiased chromatin accessibility profiling by RED-seq uncovers unique features of nucleosome variants in vivo.

ABSTRACT:

Background

Differential accessibility of DNA to nuclear proteins underlies the regulation of numerous cellular processes. Although DNA accessibility is primarily determined by the presence or absence of nucleosomes, differences in nucleosome composition or dynamics may also regulate accessibility. Methods for mapping nucleosome positions and occupancies genome-wide (MNase-seq) have uncovered the nucleosome landscapes of many different cell types and organisms. Conversely, methods specialized for the detection of large nucleosome-free regions of chromatin (DNase-seq, FAIRE-seq) have uncovered numerous gene regulatory elements. However, these methods are less successful in measuring the accessibility of DNA sequences within nucelosome arrays.

Results

Here we probe the genome-w

SUBMITTER: Chen PB 

PROVIDER: S-EPMC4378318 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature