RNA-Seq gene expression profiling of HepG2 cells: the influence of experimental factors and comparison with liver tissue.

ABSTRACT:

Background

Human hepatoma HepG2 cells are used as an in vitro model of the human liver. High-throughput transcriptomic sequencing is an advanced approach for assessing the functional state of a tissue or cell type. However, the influence of experimental factors, such as the sample preparation method and inter-laboratory variation, on the transcriptomic profile has not been evaluated.

Results

The whole-transcriptome sequencing of HepG2 cells was performed using the SOLiD platform and validated using droplet digital PCR. The gene expression profile was compared to the results obtained with the same sequencing method in another laboratory and using another sample preparation method. We also compared the transcriptomic profile HepG2 cells with that of liver tissue. Comparison of

SUBMITTER: Tyakht AV 

PROVIDER: S-EPMC4378340 | biostudies-literature | 2014 Dec

REPOSITORIES: biostudies-literature