Plant membrane assays with cytokinin receptors underpin the unique role of free cytokinin bases as biologically active ligands.

ABSTRACT: Cytokinin receptors play a key role in cytokinin-dependent processes regulating plant growth, development, and adaptation; therefore, the functional properties of these receptors are of great importance. Previously the properties of cytokinin receptors were investigated in heterologous assay systems using unicellular microorganisms, mainly bacteria, expressing receptor proteins. However, within microorganisms receptors reside in an alien environment that might distort the receptor properties. Therefore, a new assay system has been developed allowing studies of individual receptors within plant membranes (i.e. closer to their natural environment). The main ligand-binding characteristics of receptors from Arabidopsis [AHK2, AHK3, and AHK4] and maize (ZmHK1) were refined in this new system, a

SUBMITTER: Lomin SN 

PROVIDER: S-EPMC4378623 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature