Sequence variation within the KIV-2 copy number polymorphism of the human LPA gene in African, Asian, and European populations.

ABSTRACT: Amazingly little sequence variation is reported for the kringle IV 2 copy number variation (KIV 2 CNV) in the human LPA gene. Apart from whole genome sequencing projects, this region has only been analyzed in some detail in samples of European populations. We have performed a systematic resequencing study of the exonic and flanking intron regions within the KIV 2 CNV in 90 alleles from Asian, European, and four different African populations. Alleles have been separated according to their CNV length by pulsed field gel electrophoresis prior to unbiased specific PCR amplification of the target regions. These amplicons covered all KIV 2 copies of an individual allele simultaneously. In addition, cloned amplicons from genomic DNA of an African individual were sequenced. Our data suggest that s

SUBMITTER: Noureen A 

PROVIDER: S-EPMC4378929 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature