Single domain antibodies as a powerful tool for high quality surface plasmon resonance studies.

ABSTRACT: Single domain antibodies are recombinantly expressed functional antibodies devoid of light chains. These binding elements are derived from heavy chain antibodies found in camelids and offer several distinctive properties for applications in biotechnology such as small size, stability, solubility, and expression in high yields. In this study we demonstrated the potential of using single domain antibodies as capturing molecules in biosensing applications. Single domain antibodies raised against green fluorescent protein were anchored onto biosensor surfaces by using several immobilization strategies based on Ni2+:nitrilotriacetic acid-polyhistidine tag, antibody-antigen, biotin-streptavidin interactions and amine-coupling chemistry. The interaction with the specific target of the single doma

SUBMITTER: Della Pia EA 

PROVIDER: S-EPMC4378939 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature