Cryptococcus neoformans-induced macrophage lysosome damage crucially contributes to fungal virulence.

ABSTRACT: Upon ingestion by macrophages, Cryptococcus neoformans can survive and replicate intracellularly unless the macrophages become classically activated. The mechanism enabling intracellular replication is not fully understood; neither are the mechanisms that allow classical activation to counteract replication. C. neoformans-induced lysosome damage was observed in infected murine bone marrow-derived macrophages, increased with time, and required yeast viability. To demonstrate lysosome damage in the infected host, we developed a novel flow cytometric method for measuring lysosome damage. Increased lysosome damage was found in C. neoformans-containing lung cells compared with C. neoformans-free cells. Among C. neoformans-containing myeloid cells, recently recruited cells displayed lower damage

SUBMITTER: Davis MJ 

PROVIDER: S-EPMC4379045 | biostudies-literature | 2015 Mar

REPOSITORIES: biostudies-literature