Degeneration of the nonrecombining regions in the mating-type chromosomes of the anther-smut fungi.

ABSTRACT: Dimorphic mating-type chromosomes in fungi are excellent models for understanding the genomic consequences of recombination suppression. Their suppressed recombination and reduced effective population size are expected to limit the efficacy of natural selection, leading to genomic degeneration. Our aim was to identify the sequences of the mating-type chromosomes (a1 and a2) of the anther-smut fungi and to investigate degeneration in their nonrecombining regions. We used the haploid a1 Microbotryum lychnidis-dioicae reference genome sequence. The a1 and a2 mating-type chromosomes were both isolated electrophoretically and sequenced. Integration with restriction-digest optical maps identified regions of recombination and nonrecombination in the mating-type chromosomes. Genome sequence data w

SUBMITTER: Fontanillas E 

PROVIDER: S-EPMC4379399 | biostudies-literature | 2015 Apr

REPOSITORIES: biostudies-literature