Association of Intact dupA (dupA1) rather than dupA1 cluster with duodenal ulcer in Indian population.

ABSTRACT:

Background

The duodenal ulcer promoting gene (dupA) and dupA cluster in Helicobacter pylori have been described as a risk factor for duodenal ulcer development in some populations. Polymorphic gene dupA can be divided into two groups, intact dupA1 (long or short type based on the presence or absence of 615-bp extra sequences at the 5' region) having complete reading frame and other truncated dupA2 having frame-shift mutation. This study was aimed to elucidate the role of dupA of H. pylori and their clusters in the disease manifestation of Indian population.

Methods

A total of 170 H. pylori strains were screened for the presence of dupA, dupA alleles and dupA cluster by PCR and sequencing. Pro-inflammatory cytokine (IL-8) with different dupA variant H. pylori stimulated gastr

SUBMITTER: Alam J 

PROVIDER: S-EPMC4379697 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature