Functional activity of peripheral blood eosinophils in allergen-induced late-phase airway inflammation in asthma patients.

ABSTRACT:

Objective

We aimed to investigate peripheral blood eosinophil chemotaxis, generation of spontaneous reactive oxygen species (ROS), and apoptosis in patients with allergic asthma after bronchial allergen challenge.

Material and methods

A total of 18 patients with allergic asthma (AA), 14 with allergic rhinitis (AR), and 10 healthy subjects (HS) underwent bronchial challenge with a specific allergen extract. Eosinophils from peripheral blood were isolated 24 h before as well as 7 and 24 h after bronchial allergen challenge. Chemotaxis, spontaneous ROS production in eosinophils, and apoptosis were analyzed by flow cytometry. Serum and induced sputum IL-5 levels were measured by ELISA; the cell count in sputum was analyzed by the May-Grünwald-Giemsa method.

Results

Before

SUBMITTER: Lavinskiene S 

PROVIDER: S-EPMC4379944 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature