Bone tissue engineering with a collagen-hydroxyapatite scaffold and culture expanded bone marrow stromal cells.

ABSTRACT: Osteoprogenitor cells combined with supportive biomaterials represent a promising approach to advance the standard of care for bone grafting procedures. However, this approach faces challenges, including inconsistent bone formation, cell survival in the implant, and appropriate biomaterial degradation. We have developed a collagen-hydroxyapatite (HA) scaffold that supports consistent osteogenesis by donor-derived osteoprogenitors, and is more easily degraded than a pure ceramic scaffold. Herein, the material properties are characterized as well as cell attachment, viability, and progenitor distribution in vitro. Furthermore, we examined the biological performance in vivo in a critical-size mouse calvarial defect. To aid in the evaluation of the in-house collagen-HA scaffold, the in vivo pe

SUBMITTER: Villa MM 

PROVIDER: S-EPMC4380129 | biostudies-literature | 2015 Feb

REPOSITORIES: biostudies-literature