In vivo laser speckle imaging reveals microvascular remodeling and hemodynamic changes during wound healing angiogenesis.

ABSTRACT: Laser speckle contrast imaging (LSCI) is a high-resolution and high contrast optical imaging technique often used to characterize hemodynamic changes in short-term physiological experiments. In this study, we demonstrate the utility of LSCI for characterizing microvascular remodeling and hemodynamic changes during wound healing angiogenesis in vivo. A 2 mm diameter hole was made in the mouse ear and the periphery of the wound imaged in vivo using LSCI over 12 days. We were able to visualize and quantify the vascular and perfusion changes that accompanied wound healing in the microenvironment proximal to the wound, and validated these changes with histology. We found that consistent with the stages of wound healing, microvessel density increased during the initial inflammatory phase (i.e.,

SUBMITTER: Rege A 

PROVIDER: S-EPMC4380186 | biostudies-literature | 2012 Mar

REPOSITORIES: biostudies-literature