Identification of reference genes for relative quantification of circulating microRNAs in bovine serum.

ABSTRACT: Circulating microRNAs in body fluids have been implicated as promising biomarkers for physiopathology disorders. Currently, the expression levels of circulating microRNAs are estimated by reverse transcription quantitative real-time polymerase chain reaction. Use of appropriate reference microRNAs for normalization is critical for accurate microRNA expression analysis. However, no study has systematically investigated reference genes for evaluating circulating microRNA expression in cattle. In this study, we describe the identification and characterization of appropriate reference microRNAs for use in the normalization of circulating microRNA levels in bovine serum. We evaluated the expression stability of ten candidate reference genes in bovine serum by using reverse transcription quantit

SUBMITTER: Bae IS 

PROVIDER: S-EPMC4380332 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature