Substrate use of Pseudovibrio sp. growing in ultra-oligotrophic seawater.

ABSTRACT: Marine planktonic bacteria often live in habitats with extremely low concentrations of dissolved organic matter (DOM). To study the use of trace amounts of DOM by the facultatively oligotrophic Pseudovibrio sp. FO-BEG1, we investigated the composition of artificial and natural seawater before and after growth. We determined the concentrations of dissolved organic carbon (DOC), total dissolved nitrogen (TDN), free and hydrolysable amino acids, and the molecular composition of DOM by electrospray ionization Fourier transform ion cyclotron resonance mass spectrometry (ESI FT-ICR-MS). The DOC concentration of the artificial seawater we used for cultivation was 4.4 μmol C L(-1), which was eight times lower compared to the natural oligotrophic seawater we used for parallel experiments (36 μmol C

SUBMITTER: Schwedt A 

PROVIDER: S-EPMC4380363 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature