Reference gene validation for RT-qPCR, a note on different available software packages.

ABSTRACT:

Background

An appropriate normalization strategy is crucial for data analysis from real time reverse transcription polymerase chain reactions (RT-qPCR). It is widely supported to identify and validate stable reference genes, since no single biological gene is stably expressed between cell types or within cells under different conditions. Different algorithms exist to validate optimal reference genes for normalization. Applying human cells, we here compare the three main methods to the online available RefFinder tool that integrates these algorithms along with R-based software packages which include the NormFinder and GeNorm algorithms.

Results

14 candidate reference genes were assessed by RT-qPCR in two sample sets, i.e. a set of samples of human testicular tissue containing

SUBMITTER: De Spiegelaere W 

PROVIDER: S-EPMC4380439 | biostudies-literature | 2015

REPOSITORIES: biostudies-literature